Removing a codon from E. coli

The CAGE method for genome modification.

A new paper from the Church lab appears in July 15th’s issue of Science Magazine. I worked on aspects of this project during my initial rotation in the lab. In particular, I used conjugative assembly to combine together subunits of the modified genome (the CAGE method). I also led the genome re-sequencing effort. We sequenced many of the intermediate recoded strains to confirm that our changes were made successfully, to characterize the mutation rate over the replacement and assembly process, and to ensure that we did not introduce large numbers of additional point mutations or structural changes to the genome.

In addition to the paper itself, you can read popular press coverage of the research from several places:

New York Times
MIT News
And more…

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Daniel Bryan Goodman is currently an NSF Graduate Research Fellow at the Harvard-MIT Division of Health Sciences and Technology (HST). His current research involves technology development for synthetic and systems biology. He is synthesizing thousands of genes, globally modifying genomes, and interrogating the function of genetic elements in humans and model organisms for both clinical and basic research.